Year: 2020 | Month: April | Volume 10 | Issue 2
Effect of Eugenia jambolana and Psidium guajava Leaf Meal Mixture Supplementation on Performance, Biochemical Profile and Histopathological Changes of Broiler Chicks
DOI:10.30954/2277-940X.02.2020.9
Abstract:
Present study was undertaken in 120 day-old broiler chicks, randomly distributed into 4 dietary treatments (T1, T2, T3 and T4 supplemented with leaf meal mixture (LMM) of Eugenia jambolana and Psidium guajava @ 0, 2.5, 5.0 and 7.5 % of diet, respectively) each having 3 replicates (10 chicks/ replicate) in a complete randomized block design (CRD). Experimental chicks were fed basal diets (starter and finisher) supplemented with graded levels of LMM. Feed intake, body weight gain and feed conversion ratio (FCR) were recorded at weekly intervals. Two birds from each replicate were slaughtered at the end of feeding trial (42 days). Blood samples were collected and serum separated for biochemical profiles. For histopathological examination, representative tissue samples were collected in 10% neutral buffer formalin and then processed for paraffin embedding employing alcohol as dehydrating agent and xylene as clearing agent. Sections were cut at 4-5µm thickness and stained by routine haematoxylin and eosin method. On histopathological examination of liver, kidney, heart and intestine of T1 and T2 groups showed normal integrity, mild to moderate histopathological changes in T3 group, while, T4 showed drastic histopathological changes. It was concluded that E. jambolana and P. guajava LMM supplementation (2.5% or even 5%) maintained birds performance, minor histopathological changes and producing healthy low cholesterol broiler meat. The LMM incorporation (2.5%) in the diet of broiler chicks may be recommended as socioeconomic, alternative functional feed resource.
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